AKR-2B

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: mouse embryo
• packaging: tube of 5 μg 91061307-DNA-5UG; pkg of vial of cells 91061307-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Can spontaneously produce murine leukaemia virus (MLV) in prolonged confluent culture.
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse, AKR, embryo

Cell Line Description

This cell line has the capacity to be activated to produce murine leukaemia virus (MLV). Mice of the high leukaemic inbred strain AKR regularly contain high levels of MLV throughout most of their post-natal life. However, studies of the AKR mouse embryo indicated that they contain very little, or no, infectious virus. Cell lines were found to produce virus only after several weeks in culture. Virus-negative cell lines can be induced to initiate synthesis of MLV . Multiple transforming growth factors (TGFs) have been isolated from the serum-free conditioned media and acid-ethanol cell extracts of non-transformed AKR-2B cells.

Application

Culture of murine leukaemia virus

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:16 to 1:24 i.e. seeding at 4-10x1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Do not allow to reach confluence or grow over 20 passages.

Other Notes

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