WM-115

91061232, human skin, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: WM-115, 91061232
• biological source: human skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Proteoglycan antigen
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human melanoma

Cell Line Description

Derived from a primary epitheloid tumour. Expresses proteoglycan antigen characteristic of melanomas. Tumourigenic in nude mice. See also WM 266-4 (Sigma Catalogue number 91061233) which is derived from a metastasis from the same patient.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 13
D7S820: 8
THO1: 7,9
TPOX: 8,11
vWA: 15,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.