SW 837

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91031104, human colon, Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: human colon
• packaging: tube of 5 μg 91031104-DNA-5UG; pkg of vial of cells 91031104-1VL
• growth mode: Adherent
• karyotype: 2n = 46, hypodiploid
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian rectum adenocarcinoma

Cell Line Description

Established from a Grade IV adenocarcinoma of the rectum of a 53 year old Caucasian male. The cells produce CEA and electron microscopy reveals brush borders. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,9
vWA: 15,16

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; No CO₂; 37°C. After thawing the 1st subculture interval may be 14-18 days. Medium change after 4 days.

Other Notes

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