Detroit 539

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human skin
• packaging: tube of 5 μg 91031102-DNA-5UG; pkg of vial of cells 91031102-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• Storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian skin Down syndrome

Cell Line Description

Derived from a female caucasian infant with Down Syndrome. Karyotypic analysis shows 90% of the cell population possesses 47 chromosomes with 5 chromosomes belonging to G group. These findings persist essentially unchanged throughout at least 30 serial subcultures.

Application

Chromosome studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 11,13
D16S539: 13
D5S818: 11,13
D7S820: 9,11
THO1: 8,9
TPOX: 8,10
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. A further 15-20 passages expected.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.