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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
opossum kidney
packaging
tube of 5 μg 91021202-DNA-5UG
pkg of vial of cells 91021202-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Insulin, serotonin, alpha 2-adrenergic parathy. Hormone
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
American Opossum, kidney
Cell Line Description
Derived from proximal tubule of a kidney taken from an adult female opossum. Cells retain proximal tubule function. Possesses the following receptors: α 2 - adrenergic, insulin, serotonin, parathyroid hormone and atrial natriuretic factor.
Application
Kidney function studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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