EB-3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human unknown/unspecified
• packaging: tube of 5 μg 90121003-DNA-5UG; pkg of vial of cells 90121003-1VL
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Burkitt′s lymphoma

Cell Line Description

The cell line EB3 has been established in 1964 from a 3 year old male with Burkitt′s lymphoma. Herpes-like virus particles have been detected by electron microscopy. Antibodies from patients with infectious mononucleosis react with the herpes type virus (EBV) found in EB3 cells. The cell line has been shown to be resistant to challenge with vesicular stomatitis virus. Lymphoblastoid, grow as single cells and in clumps. Ethnicity: Black.

Application

Study of Burkitt′s lymphoma, virus sensitivity

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO₂; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.