C211

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human skin
• packaging: tube of 5 μg 90112604-DNA-5UG; pkg of vial of cells 90112604-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian skin, Cri du Chat syndrome

Cell Line Description

Derived from a biopsy of an 11 year old Caucasian male with Cri du Chat syndrome. Cells have a limited lifespan of approximately 35 passages. Autoradiographic studies demonstrated that a distinction could be made between chromosome 4 and 5 in the Cri du chat syndrome by alternative DNA replication pattern and length of long and short arms.

Application

Genetic studies

Culture Medium

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.