Jensen sarcoma, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 90111913
biological source
rat unknown/unspecified
packaging
tube of 5 μg 90111913-DNA-5UG pkg of vial of cells 90111913-1VL
Used in studies relating to amino acid requirements and L-asparagine metabolism. Cloning studies show that variants not requiring L-asp are present in 100 per 1,000,000 cells Cloned populations which do not produce variants immediately do so after 6-8 further passages.
Renew medium daily where possible. Subculture by placing culture in cold room (4°C) for 60 minutes, shake flask to suspend cells, centrifuge, resuspend in fresh medium and into fresh flasks. Split sub-confluent cultures (70-80%) 1:2 to 1:10; 5% CO2; 37°C.
Other Notes
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