G-7
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
mouse muscle
packaging
tube of 5 μg 90110520-DNA-5UG
pkg of vial of cells 90110520-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Myoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Storage temp.
−196°C
Cell Line Origin
Mouse Swiss-Webster myoblast
Cell Line Description
Derived from a muscle tissue culture of a foetal Swiss-Webster mouse. Tumorigenic and only slightly sensitive to acetylcholine. When confluent cultures form short multinucleated myotubes.
Application
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin in Ca2+ + Mg2+-free PBS; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.
Other Notes
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