Citrullinemia

90102532, human skin, Fibroblast

• UNSPSC Code: 41106514

Properties

• Product Name: Citrullinemia, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 90102532
• biological source: human skin
• packaging: tube of 5 μg 90102532-DNA-5UG; pkg of vial of cells 90102532-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian skin

Cell Line Description

Human diploid cell line derived from a young Caucasian female with Citrullinemia. The cells grow poorly when citrulline is substituted for arginine in the recommended medium and they incorporate very little 14C citrulline into cell protein. The cell line also has an abnormally low affinity for citrulline as a substrate in an in vivo assay of arginosuccinate synthetase.

Application

Genetic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Finite lifespan up to 30 passages.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.