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product name
3T3-MSV, 90092701
biological source
mouse embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Growth factor
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
General description
Product has lost contact inhibition
Cell Line Origin
Mouse Swiss 3T3 embryo, Moloney-MSV transformed
Cell Line Description
This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.
Application
Cell growth studies
Culture Medium
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.
Other Notes
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Certificates of Analysis (COA)
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