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OX-47

Reactivity: Anti-Lymphocytes (activated), rat, 90071611

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About This Item

UNSPSC Code:
12352207
Pricing and availability is not currently available.

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

NS0/1

Reactivity

Anti-Lymphocytes (activated), rat

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

General description

For complete product information, please see OX-47

Cell Line Origin

Mouse NS0/1 x Mouse BALB/c spleen

Cell Line Description

The antibody binds to activated rat lymphocytes. It labels all T and B cell blasts strongly, gives -ve to weak labelling of thoracic duct lymphocytes and thymocytes. The antibody labels vascular endothelium, kidney tubules and brain capillaries. Endothelium of large vessels do not react.

Immunogen

Rat T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.

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