CCD-18Co

90070503, human colon, Fibroblast

• UNSPSC Code: 12352200

Properties

• Product Name: CCD-18Co, 90070503
• biological source: human colon
• growth mode: Adherent
• karyotype: modal No 46
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human colon fibroblast, normal, ethnicity: Black

Cell Line Description

Derived from biopsy colon tissue of a black female infant (2.5 months old). CCD 18Lu (90112706) and CCD-18Co (90070503) were derived from the lung and colon respectively from the same patient; this has been confirmed by STR profiling at ECACC.

Application

Aging studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 8
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 8
THO1: 6,7
TPOX: 8,11
vWA: 15,17

Culture Medium

EMEM (EBSS) + 2mM glutamine + 1% NEAA + 10% FBS

Subculture Routine

Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.