MSVIF-TK+
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
mouse embryo
packaging
tube of 5 μg 90031902-DNA-5UG
pkg of vial of cells 90031902-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/c embryonic fibroblast, M-MSV transformed
Cell Line Description
Derived from the MSV.B line (Sigma Catalogue number. 90031901) which has been exposed to 50 IU/ml Interferon for over 200 passages. Unlike the parent line, the cells are non-malignant and do not form tumours in nude mice.
Application
Interferon, transformation and virus studies: M-MSV
Culture Medium
EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) or RPMI 1640 + 2mM Glutamine + 50-100 IU/ml murine IFN + 5-10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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