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Product Name
N1-S1, 90011902
biological source
rat liver
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat hepatoma
Cell Line Description
Established from the ascitic fluid of a Novikoff hepatoma. The cells can be synchronised for cell cycle studies. N1-S1 has also been used for studies of pyrimidine metabolism, DNA synthesis and methylation and ribosomal RNA maturation.
Application
Cell cycle, metabolic and mutation studies
Culture Medium
Swimm′s S-77 + 2mM Glutamine + 0.1% Pluronic F68 +10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 4x10,000 to 1,000,000 cells/ml; 5% CO2; 37°C. Subculture every 3-4 days by centrifugation or medium addition.
Other Notes
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