153BR, 90011808, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human skin
packaging
tube of 5 μg 90011808-DNA-5UG pkg of vial of cells 90011808-1VL
Established from a biopsy of a normal 56 year old male. The cells have been used in radiation survival studies. EBV-transformed lymphocytes designated LB185 (Sigma Catalogue number. 98062318) have been isolated from the same individual. This has been confirmed by STR profiling at ECACC.
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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