Product Name
Sp1K, 89120705
biological source
dolphin kidney
growth mode
Adherent
karyotype
2n = 44
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Dolphin kidney
Cell Line Description
From adult male Atlantic spotted dolphin (Stenella plagiodon). Susceptible to wide range of terrestrial mammalian viruses: Ad5, Coxsackie A9+B5, Reovirus3, Vaccinia VSV (Ogden strain), BVD, IBR, Influenza A, parainfluenza3, hog cholera (Ames strain), Western equine encephalitis, St Louis encephalitis and Powassan viruses. Also known as Sp1K (NBL-10).
Application
Virus studies: a wide range of mammalian viruses
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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