NC-37

89111414, human blood, Lymphoblast

• UNSPSC Code: 12352207

Properties

• Product Name: NC-37, 89111414
• biological source: human blood
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian peripheral blood lymphocyte

Cell Line Description

Derived from peripheral blood of a normal 34 year male Caucasian. Each cell has an average 60 EBV genome copies. Cells have been used in EBV superinfection studies in addition to chemical induction of the EBV genome. They are EBNA positive. The cells should be handled under laboratory containment level 2.

Application

Virus studies: EBV, oncornavirus propagation

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 4-5x100,000 cells/ml; Maximum cell density is 2x1,000,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.