U373 MG Cell Line human, 89081403, Isolated from brain epithelium
biological source
human brain
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Derived from a malignant tumor by explant technique. Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4 × 104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA; 37 °C and 5% CO2. Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 1 mM Sodium pyruvate + 10% FBS.
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