ICR-2A

89072615, frog embryo, Fibroblast-like

• UNSPSC Code: 41106514

Properties

• Product Name: ICR-2A, 89072615, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: frog embryo
• packaging: tube of 5 μg 89072615-DNA-5UG; pkg of vial of cells 89072615-1VL
• growth mode: Adherent
• karyotype: 2n = 26, Stable haploid
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Frog Grass embryo

Cell Line Description

Previously designated RPH68.2A. Derived from androgenetic haploid embryos of the grass or leopard frog, (Rana pipiens). The cells retain their haploid chromosome complement for over 200 generations. Used in genetic studies.

Application

Genetic studies

Culture Medium

L15 (50%) + Distilled Water (40%) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 25°C. Do not use CO₂.Grow cells in dark (i.e wrapped in foil). Cells do not reach 100% confluency, only approximately 70-80%. Freeze in glycerol.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.