MN4-91-6.E2

89062111, Reactivity: Anti-RT1-A MHC class I antigen (polymorphic determinant), rat, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 89062111-DNA-5UG; pkg of vial of cells 89062111-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS1/1
• Reactivity: Anti-RT1-A MHC class I antigen (polymorphic determinant), rat
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse NS1/1 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against a polymorphic determinant on the rat MHC Class I antigens. It shows a high degree of allospecificity, reacting with DA, PVG RT1a and PVG RT1rl rats, but not PVG, WAG or LEW rats.

Immunogen

DA rat, purified MHC class I antigen

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder cells upon resuscitation

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.