F8-11-13

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 89062102, Reactivity: Anti-CD45R, human

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 89062102-DNA-5UG; pkg of vial of cells 89062102-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS1/1
• Reactivity: Anti-CD45R, human
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• Storage temp.: −196°C

Description

Cell Line Origin

Mouse NS1/1 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against CD45R, which is selectively expressed principally on B lymphocytes. A small proportion of thymocytes are weakly positive and granulocytes are negative.

Immunogen

Fresh human lymph node lymphocytes depleted of B lymphocytes

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder cells upon resuscitation

Other Notes

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