Bu (IMR-31)

89051704, buffalo lung, Fibroblast

• UNSPSC Code: 41106514

Properties

• Product Name: Bu (IMR-31), NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 89051704
• biological source: buffalo lung
• packaging: tube of 5 μg 89051704-DNA-5UG; pkg of vial of cells 89051704-1VL
• growth mode: Adherent
• karyotype: 2n = 60
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Buffalo lung

Cell Line Description

Derived from female Buffalo yearling. The cells have a finite life span of a minimum of 30 passages and are susceptible to a range of viruses including HSV, vaccinia, PRV, BVDV, para-influenza 3, bovine enterovirus (M62/4); also IBRV and VSV (Indiana). The cell line produces both detectable BVDV antigens and infectious BVDV.

Application

Virus replication studies

Culture Medium

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.