C-4I

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human cervix
• packaging: tube of 5 μg 89051702-DNA-5UG; pkg of vial of cells 89051702-1VL
• growth mode: Adherent
• karyotype: 2n = 46, hypodiploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian Cervical carcinoma

Cell Line Description

Established from a cervical carcinoma of a 41 year old Caucasian female. Cells form stratified, epithelial colonies and retain properties of squamous cell differentiation in long term culture. They are morphologically and cytogenetically distinct from C-4 II (Sigma Catalogue number. 88102701). Reported to contain human papilloma virus. Handle as potentially biohazardous material.

Application

Differentiation studies

Culture Medium

W aymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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