Used for propagation of several viruses, including St Louis, Eastern Equine and California encephalitis; duck virus enteritis agent; also for preparation of live rubella virus vaccine.
Application
Vaccine development and virus replication studies: rubella, duck enteritis virus
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. At least 30 passages.
Other Notes
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