G-8
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106514
biological source
mouse muscle
packaging
tube of 5 μg 89050906-DNA-5UG
pkg of vial of cells 89050906-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Myoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Swiss-Webster myoblast
Cell Line Description
Isolated from a myogenic line derived from hind limb muscle of a foetal Swiss-Webster mouse. Non-tumorigenic and highly sensitive to acetylcholine. Confluent cultures form multinucleated myotubes.
Culture Medium
DMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service