ECACC routinely tests all manufactured cell banks for mycoplasma.
Fao Cell Line from rat
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89042701, rat liver, Hepatocyte
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
rat liver
packaging
tube of 5 μg 89042701-DNA-5UG
pkg of vial of cells 89042701-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Hepatocyte
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat hepatoma
Cell Line Description
A differentiated cell line derived from H4-11-E-C3 (Sigma catalogue no. 85061112). A versatile tool for studying regulation of mono-oxygenase activities and analysing their role in the activation of xenobiotics e.g. carcinogens, drugs and pesticides.
Application
Differentiation and enzymatic studies
Culture Medium
Coon′s modified Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or kaign′s modified Ham′s F12 + 45mg/l asorbic acid +18mg/1 inositol + 10% FBS +2mM Glutamine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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Are my Cell Line cells mycoplasma tested?
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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