YTS 156.7.7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-Lyt-3 epitope B, mouse, 89040604

• UNSPSC Code: 41106514

Properties

• biological source: Rat x Rat Hybridoma
• packaging: tube of 5 μg 89040604-DNA-5UG; pkg of vial of cells 89040604-1VL
• ImmunoDon: DA rat spleen
• Myeloma: Y3Ag1.2.3
• Reactivity: Anti-Lyt-3 epitope B, mouse
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2b
• shipped in: dry ice
• Storage temp.: −196°C

Description

Cell Line Description

The antibody is directed against the B epitope of the mouse Lyt-3 (CD8) antigen.Has been used in CD8 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB15: www.enterprise.cam.ac.uk.

Immunogen

CBA mouse thymocytes

Culture Medium

DMEM + 2 mM glutamine + 0.05 mM 2-mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9 x 10⁵ cells/ml. 5% CO₂; 37 °C. Saturation density 9 x 10⁵/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.