C-4 II
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
human cervix
packaging
tube of 5 μg 88102701-DNA-5UG
pkg of vial of cells 88102701-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian cervical carcinoma
Cell Line Description
From a cervical carcinoma of a 41 year old Caucasian female. Cells form spreading predominantly monolayered, epithelial colonies with hemicysts which are characteristic of transporting epithelia and respond to crowding by separation and shedding. These cells have retained properties of basal cell differentiation in long term culture and exhibit G6PD type B. C-4 II cells have been reported to contain human papilloma virus 18 (HPV-18) DNA sequences and to express HPV-18 RNA.
Application
Differentiation studies
Culture Medium
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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