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OX-41

88051304, Reactivity: Anti-Macrophage subset, rat

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

NS0/U

Reactivity

Anti-Macrophage subset, rat

technique(s)

cell culture | mammalian: suitable

isotype

IgG2a

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse NS0/U x Mouse BALB/c spleen

Cell Line Description

The antibody recognises a rat macrophage cell surface antigen and labels all peritoneal and 90% of alveolar macrophages. Splenic red pulp macrophages are antigen +ve, marginal zone macrophages antigen -ve. The antibody also reacts with the 120kDa antigen on granulocytes and dendritic cells and recognises rat SIRP (personal communication).

Immunogen

Resident peritoneal cells from (PVG-RT1c x PVG RT1u)F1 and (PVG-RT1c x DA-RT1a)F1 rats

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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