NFS-5 C1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
mouse blood
packaging
tube of 5 μg 88041904-DNA-5UG
pkg of vial of cells 88041904-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Ecotropic and mink cell focus-forming viruses
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse pre-B lymphoblast
Cell Line Description
Established from a lymphoma produced in an NFS/N mouse by injecting CaS-25M ecotropic MuLV. Cells show markers of large pre-B lymphoblasts and spontaneously produce ecotropic and mink cell focus-forming viruses.
Application
Virus studies: MuLV, ecotropic and focus-forming viruses
Culture Medium
DMEM + 2mM Glutamine + 1mM Oxaloacetic acid + 0.2U/ml Bovine Insulin + 2ug/ml Transferrin + 50μM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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