AFRC IAH-CC15
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-WC1, bovine, 88022410
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 88022410-DNA-5UG
pkg of vial of cells 88022410-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1
Reactivity
Anti-WC1, bovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS1 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against WC1 antigen found on bovine CD4- CD8- and CD2- T cells which express gamma/delta TcR. The antigen has a molecular weight of 215-300kDa and is equivalent to the T19 antigen in sheep. The antibody is directed against equivalent antigen in sheep and goats.
Immunogen
Bovine lymphocytes
Application
Flow cytometry, immunohistology, immunoprecipitation, complement lysis, in vivo depletion
Culture Medium
RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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