IEC 18
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88011801, rat intestine, Epithelial
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
rat intestine
packaging
tube of 5 μg 88011801-DNA-5UG
pkg of vial of cells 88011801-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Storage temp.
−196°C
Cell Line Origin
Normal rat ileum
Cell Line Description
Derived from normal epithelial cells of the rat ileum. Cells should undergo at least 20 population doublings. The line was established using the same techniques as for IEC6 (Sigma Catalogue number. 88071401). IEC 18 cells grow in small islands and do not reach confluency.
Application
Small bowel biology
Culture Medium
DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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