Derived from the liver of a Wistar rat. Useful in quantitative carcinogenesis studies. Inoculation into new born rats is more sensitive than nude mice for detection of tumorigenicity of rat liver lines.
Split sub-confluent cells 1:3 to 1:6 i.e. 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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