BF-2
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
fish trunk
packaging
tube of 5 μg 87032603-DNA-5UG
pkg of vial of cells 87032603-1VL
growth mode
Adherent
karyotype
2n = 48
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Fish Bluegill fry caudal trunk
Cell Line Description
Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15°C to 33°C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.
Application
Virus studies, targets for bacterial cytotoxins
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO2; 23°C. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells
Other Notes
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