Detroit 532

87032602, human skin, Fibroblast

• UNSPSC Code: 41106514

Properties

• Product Name: Detroit 532, 87032602, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: human skin
• packaging: tube of 5 μg 87032602-DNA-5UG; pkg of vial of cells 87032602-1VL
• growth mode: Adherent
• karyotype: 2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian, skin, Down syndrome

Cell Line Description

Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.

Application

Virus studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, up to 30 passages expected.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.