L-M, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 87032401
biological source
mouse connective tissue
packaging
tube of 5 μg 87032401-DNA-5UG pkg of vial of cells 87032401-1VL
Derived from NCTC clone 929 in serum-free medium. The cells were adapted from horse serum containing medium to serum-free over a period of one year.
Application
Genetic and biochemical studies
Culture Medium
Medium 199 + 2mM Glutamine + 0.5% Bacto-peptone (Difco).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C.
Other Notes
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