SW 13

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human adrenal gland (cortex)
• packaging: tube of 5 μg 87031801-DNA-5UG; pkg of vial of cells 87031801-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian adrenal cortex adenocarcinoma

Cell Line Description

Derived from biopsy tissue of a small cell carcinoma originating in the adrenal cortex of a 55 year old Caucasian female. Electron microscopy revealed bulb gap junctions and occasional paired cisternae of the E.R.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 12
D7S820: 8,10
THO1: 7,8
TPOX: 8
vWA: 17,19

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C.

Other Notes

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