LS180

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021202, human colon, Epithelial-like

• UNSPSC Code: 41106514

Properties

• biological source: human colon
• packaging: tube of 5 μg 87021202-DNA-5UG; pkg of vial of cells 87021202-1VL
• growth mode: Adherent
• karyotype: Modal no. 45 (2n=46)
• morphology: Epithelial-like
• products: Carcinoembryonic antigen (CEA), Mucin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• Storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Application

Tumourigenicity studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

Other Notes

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