Derived from the human lymphoblast TK6, which is heterozygous for TK. The parent line was exposed to 20ug/ml thioguanine which produced a thioguanine resistant sub-population. The cells can be used as a fusion partner.
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
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