NRK-52E
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012902, rat kidney, Epithelial
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
rat kidney
packaging
tube of 5 μg 87012902-DNA-5UG
pkg of vial of cells 87012902-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat kidney epithelial
Cell Line Description
Cloned from the same mixed culture of normal rat kidney cells as NRK-49F, but has epithelial-like morphology. It has distinct growth and transforming characteristics distinct from NRK-49F.
Application
NRK-52E has been used to study the effects of altered selenium supply on the expression of endogenous thioredoxin reductase (TrxR).[1]
Culture Medium
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Remove all traces of trypsin from cultures during sub-culturing i.e. spin cells or neutralise with serum.
Other Notes
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