AFRC MAC 79

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-Nitrate Reductase, 86112691

• UNSPSC Code: 41106514

Properties

• biological source: Rat x Rat Hybridoma
• packaging: tube of 5 μg 86112691-DNA-5UG; pkg of vial of cells 86112691-1VL
• ImmunoDon: F344 rat spleen
• Myeloma: YB2/3.0 Ag20
• Reactivity: Anti-Nitrate Reductase
• technique(s): cell culture | mammalian: suitable
• isotype: IgG
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat YB2/3.0 Ag20 x Rat F344 spleen

Cell Line Description

The antibody is specific for NADH-nitrate reductase of spinach and marrow. It inhibits the reduction of nitrate to nitrite using NADH as elector donor and also inhibits dehydrogenase activity of NADH - Cytochrome c reductase.

Immunogen

Nitrate reductase purified from the leaves of 6-8 week old spinach plants - Spinacea deracea

Application

Affinity purification of nitrate reductase

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse feeder layers upon resuscitation

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.