KG-1

86111306, human bone marrow, Myeloblast-like

• Synonym(s): KG1 Cells
• UNSPSC Code: 41106514

Properties

• product name: KG-1, 86111306
• biological source: human bone marrow
• growth mode: Suspension
• karyotype: 2n = 46, diploid
• morphology: Myeloblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian bone marrow myelogenous leukaemia

Cell Line Description

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Application

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Culture Medium

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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