CCD 16Lu

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human lung
• packaging: tube of 5 μg 86090301-DNA-5UG; pkg of vial of cells 86090301-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian lung

Cell Line Description

Established from normal lung tissue of a 35 year old Caucasian male who died of a class 1-2 astrocytoma. The donor who was maintained for organ transplantation prior to removal of the lung. The cells will undergo a further 11 doublings.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 13
D5S818: 11,13
D7S820: 7,11
THO1: 9
TPOX: 10,11
vWA: 18,20

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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