3.334 SC5/8
86082107, Mouse x Rat Hybridoma hybrid, Fibroblast
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About This Item
UNSPSC Code:
41106514
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Product Name
3.334 SC5/8, 86082107
biological source
Mouse x Rat Hybridoma hybrid
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat x Mouse hybrid
Cell Line Description
Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35°C but not at 39.5°C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.
Application
Cellular control studies of neo-plastic transformation
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 35°C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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