II b
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
Human x Mouse Hybridoma unknown/unspecified
packaging
tube of 5 μg 86072805-DNA-5UG
pkg of vial of cells 86072805-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse/human hybrid
Cell Line Description
The hybrid cell line II b has been established by transforming mouse NIH 3T3 (ECACC catalogue no. 93061524) with mitotic chromosomes from the human cell line MNNG-HOS (Sigma Catalogue number. 87070201). Various probes showed stable integration of chromosome 7 DNA (7q22-31.3 and 7q22-7 cen) being closely linked to the gene mutation causing cystic fibrosis
Application
Cystic fibrosis studies, diagnostic assays
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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