DF
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
biological source
Human x Mouse Hybridoma unknown/unspecified
packaging
tube of 5 μg 86072802-DNA-5UG
pkg of vial of cells 86072802-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse / Human Hybrid, NIH-3T3 transfectant
Cell Line Description
The cell line DF has been derived by transfecting NIH-3T3 with human chromosome 7 DNA. It was originally established for the analysis of the cystic fibrosis locus and contains a transgenomic size of 3-4 Mb. Chromosomal fragments detected were 7q22-31.3 (met oncogene) and 7q22-7cen.
Application
Analysis of human chromosome 7 transfectants.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5%CO2; 37°C.
Other Notes
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