AFRC MAC 63
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 86030311-DNA-5UG
pkg of vial of cells 86030311-1VL
ImmunoDon
DA rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Peribacteroid membrane from Pisum sativum
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
Storage temp.
−196°C
Cell Line Origin
Rat Y3Ag1.2.3 x Rat DA spleen
Cell Line Description
The antibody is directed against peribacteroid material from Rhizobium-induced root nodules of Pisum sativum. The antigen is also present on plasma membranes and in the Golgi bodies, but not in endoplasmic reticulum (ER). The antibody also reacts with peribacteroid membranes from nodules of Vicia and lupin.
Immunogen
Peribacteroid material followed by peribacteroid material depleted of bacterial lipopolysaccharide
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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