MH1C1

85112702, rat liver, Epithelial

• UNSPSC Code: 12352207

Properties

• Product Name: MH1C1, 85112702
• biological source: rat liver
• growth mode: Adherent
• karyotype: Modal no. 48; hyperdiploid
• morphology: Epithelial
• products: Rat serum albumin, tyrosine aminotransferase
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat liver hepatoma

Cell Line Description

Derived from the Morris Hepatoma but is distinct from HTC cell line (Sigma Catalogue number. 93120108). Immunological experiments demonstrate that the cultures synthesise and secrete rat serum albumin and synthesise tyrosine aminotransferase. Functions of the cells include the conjugation and metabolism of testosterone and bilirubin and the production of serum complement component, especially C9. Epithelial cells forming compact smooth bordered colonies.

Application

Hormone and Enzymatic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or Ham′s F10 + 10% Horse Serum (HS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.