H129-19.6.8

85103101, Reactivity: Anti-L3T4, mouse, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Rat Heterohybridoma
• packaging: tube of 5 μg 85103101-DNA-5UG; pkg of vial of cells 85103101-1VL
• ImmunoDon: LOU/WS1 rat spleen
• Myeloma: P3X63Ag8.653
• Reactivity: Anti-L3T4, mouse
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a, kappa
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse P3X63Ag8.653 x Rat LOU/WS1 spleen

Cell Line Description

The antibody recognises a monomorphic determinant on the L3T4 molecule (the murine equivalent of CD4).

Immunogen

Lyt-1+2- anti-I-Ak cytolytic T lymphocytes

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.